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pp2b aβ sirna  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology pp2b aβ sirna

    Pp2b Aβ Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pp2b+a%CE%B2/PP2B-A%CE%B2+siRNA/pmc11407306-51-0-3
    Average 93 stars, based on 4 article reviews
    pp2b aβ sirna - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Calcineurin is an adaptor required for assembly of the TCR signaling complex"

    Article Title: Calcineurin is an adaptor required for assembly of the TCR signaling complex

    Journal: Cell reports

    doi: 10.1016/j.celrep.2024.114568


    Figure Legend Snippet:

    Techniques Used: Purification, Recombinant, Protease Inhibitor, Cell Recovery, In Situ, Plasmid Preparation, Control, Software, Luminex

    Related Articles

    Blocking Assay:

    Article Title: Effects of Single-Dose and Long-Term Ketamine Administration on Tau Phosphorylation-Related Enzymes GSK-3β, CDK5, PP2A, and PP2B in the Mouse Hippocampus.
    Article Snippet: Ketamine is a recreational drug that causes emotional and cognitive impairments, but its specific mechanisms of action are still unclear.. Recent evidence suggests that Tau protein phosphorylation and targeted delivery to the postsynaptic area are closely related to its neurotoxicity, and our recent studies have shown that long-term ketamine administration causes excessive Tau protein phosphorylation.. However, the regulatory mechanism of Tau protein phosphorylation induced by ketamine has not been clarified.

    Saline:

    Article Title: Effects of Single-Dose and Long-Term Ketamine Administration on Tau Phosphorylation-Related Enzymes GSK-3β, CDK5, PP2A, and PP2B in the Mouse Hippocampus.
    Article Snippet: Ketamine is a recreational drug that causes emotional and cognitive impairments, but its specific mechanisms of action are still unclear.. Recent evidence suggests that Tau protein phosphorylation and targeted delivery to the postsynaptic area are closely related to its neurotoxicity, and our recent studies have shown that long-term ketamine administration causes excessive Tau protein phosphorylation.. However, the regulatory mechanism of Tau protein phosphorylation induced by ketamine has not been clarified.

    Incubation:

    Article Title: Effects of Single-Dose and Long-Term Ketamine Administration on Tau Phosphorylation-Related Enzymes GSK-3β, CDK5, PP2A, and PP2B in the Mouse Hippocampus.
    Article Snippet: Ketamine is a recreational drug that causes emotional and cognitive impairments, but its specific mechanisms of action are still unclear.. Recent evidence suggests that Tau protein phosphorylation and targeted delivery to the postsynaptic area are closely related to its neurotoxicity, and our recent studies have shown that long-term ketamine administration causes excessive Tau protein phosphorylation.. However, the regulatory mechanism of Tau protein phosphorylation induced by ketamine has not been clarified.

    Control:

    Article Title: Effects of Single-Dose and Long-Term Ketamine Administration on Tau Phosphorylation-Related Enzymes GSK-3β, CDK5, PP2A, and PP2B in the Mouse Hippocampus.
    Article Snippet: Ketamine is a recreational drug that causes emotional and cognitive impairments, but its specific mechanisms of action are still unclear.. Recent evidence suggests that Tau protein phosphorylation and targeted delivery to the postsynaptic area are closely related to its neurotoxicity, and our recent studies have shown that long-term ketamine administration causes excessive Tau protein phosphorylation.. However, the regulatory mechanism of Tau protein phosphorylation induced by ketamine has not been clarified.

    Article Title: Ehrlichia chaffeensis Exploits Canonical and Noncanonical Host Wnt Signaling Pathways To Stimulate Phagocytosis and Promote Intracellular Survival
    Article Snippet: The lipid raft-disrupting agent nystatin was from Sigma. .. Human DKK3, Dvl2, Fzd9, Jun, NFATC1 (NFAT2), NFATC3 (NFAT4), PP2B-Aα (calcineurin PPP3CA), PP2B-Aβ (calcineurin PPP3CB), TCF4, Wnt6, Wnt10a, and control-A siRNAs were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). .. Validated siRNAs of human β-catenin, Wnt3a, Wnt5a, and LRP6 and endoribonuclease-prepared siRNAs (esiRNAs) of human ARID1B, KDM6B, IRF2BP2, PPP3R1, and VPS29 were from Sigma.



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    Figure 7. LRRK2G2019S-mediated TFEB nuclear translocation requires Ca2+, NAADP, and TPCN2 (A) Confocal imaging in HeLa cells expressing TFEB-mCherry and a control vector (MT) or His-LRRK2G2019S with or without BAPTA-AM (fast calcium chelator, 10 μM, 1 h), EGTA-AM (slow calcium chelator, 10 μM, 1 h), FK506 (calcineurin inhibitor, 5 nM, 1 h), control siRNA, <t>PPP3CB</t> siRNA, or MCOLN1 siRNA. Scale bar: 10 μm. (B) Quantification TFEB nuclear translocation from (A) (n = 3, ≥ 50 cells/repeat). (C) Confocal imaging in HeLa cells expressing TFEB-mCherry and a control vector (MT), or TFEB-mCherry and His-LRRK2G2019S with or without Ned-19 (NAADP inhibitor, 100 μM, 1 h), PERK inhibitor (PERK-I, 10 μM, 1 h), control siRNA, or TPCN2 siRNA. Scale bar: 10 μm. (D) Quantification of TFEB nuclear translocation from (C) (n = 3, ≥ 50 cells/repeat). (E) Immunoblot of endogenous TFEB from nuclear fractions of HEK293T cells transfected with a control vector or His-LRRK2G2019S
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    Figure 7. LRRK2G2019S-mediated TFEB nuclear translocation requires Ca2+, NAADP, and TPCN2 (A) Confocal imaging in HeLa cells expressing TFEB-mCherry and a control vector (MT) or His-LRRK2G2019S with or without BAPTA-AM (fast calcium chelator, 10 μM, 1 h), EGTA-AM (slow calcium chelator, 10 μM, 1 h), FK506 (calcineurin inhibitor, 5 nM, 1 h), control siRNA, <t>PPP3CB</t> siRNA, or MCOLN1 siRNA. Scale bar: 10 μm. (B) Quantification TFEB nuclear translocation from (A) (n = 3, ≥ 50 cells/repeat). (C) Confocal imaging in HeLa cells expressing TFEB-mCherry and a control vector (MT), or TFEB-mCherry and His-LRRK2G2019S with or without Ned-19 (NAADP inhibitor, 100 μM, 1 h), PERK inhibitor (PERK-I, 10 μM, 1 h), control siRNA, or TPCN2 siRNA. Scale bar: 10 μm. (D) Quantification of TFEB nuclear translocation from (C) (n = 3, ≥ 50 cells/repeat). (E) Immunoblot of endogenous TFEB from nuclear fractions of HEK293T cells transfected with a control vector or His-LRRK2G2019S
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    Image Search Results


    Journal: Cell reports

    Article Title: Calcineurin is an adaptor required for assembly of the TCR signaling complex

    doi: 10.1016/j.celrep.2024.114568

    Figure Lengend Snippet:

    Article Snippet: PP2B-Aβ siRNA , Santa Cruz , Cat# sc-39195.

    Techniques: Purification, Recombinant, Protease Inhibitor, Cell Recovery, In Situ, Plasmid Preparation, Control, Software, Luminex

    Figure 7. LRRK2G2019S-mediated TFEB nuclear translocation requires Ca2+, NAADP, and TPCN2 (A) Confocal imaging in HeLa cells expressing TFEB-mCherry and a control vector (MT) or His-LRRK2G2019S with or without BAPTA-AM (fast calcium chelator, 10 μM, 1 h), EGTA-AM (slow calcium chelator, 10 μM, 1 h), FK506 (calcineurin inhibitor, 5 nM, 1 h), control siRNA, PPP3CB siRNA, or MCOLN1 siRNA. Scale bar: 10 μm. (B) Quantification TFEB nuclear translocation from (A) (n = 3, ≥ 50 cells/repeat). (C) Confocal imaging in HeLa cells expressing TFEB-mCherry and a control vector (MT), or TFEB-mCherry and His-LRRK2G2019S with or without Ned-19 (NAADP inhibitor, 100 μM, 1 h), PERK inhibitor (PERK-I, 10 μM, 1 h), control siRNA, or TPCN2 siRNA. Scale bar: 10 μm. (D) Quantification of TFEB nuclear translocation from (C) (n = 3, ≥ 50 cells/repeat). (E) Immunoblot of endogenous TFEB from nuclear fractions of HEK293T cells transfected with a control vector or His-LRRK2G2019S

    Journal: Autophagy

    Article Title: LRRK2 is required for CD38-mediated NAADP-Ca 2+ signaling and the downstream activation of TFEB (transcription factor EB) in immune cells.

    doi: 10.1080/15548627.2021.1954779

    Figure Lengend Snippet: Figure 7. LRRK2G2019S-mediated TFEB nuclear translocation requires Ca2+, NAADP, and TPCN2 (A) Confocal imaging in HeLa cells expressing TFEB-mCherry and a control vector (MT) or His-LRRK2G2019S with or without BAPTA-AM (fast calcium chelator, 10 μM, 1 h), EGTA-AM (slow calcium chelator, 10 μM, 1 h), FK506 (calcineurin inhibitor, 5 nM, 1 h), control siRNA, PPP3CB siRNA, or MCOLN1 siRNA. Scale bar: 10 μm. (B) Quantification TFEB nuclear translocation from (A) (n = 3, ≥ 50 cells/repeat). (C) Confocal imaging in HeLa cells expressing TFEB-mCherry and a control vector (MT), or TFEB-mCherry and His-LRRK2G2019S with or without Ned-19 (NAADP inhibitor, 100 μM, 1 h), PERK inhibitor (PERK-I, 10 μM, 1 h), control siRNA, or TPCN2 siRNA. Scale bar: 10 μm. (D) Quantification of TFEB nuclear translocation from (C) (n = 3, ≥ 50 cells/repeat). (E) Immunoblot of endogenous TFEB from nuclear fractions of HEK293T cells transfected with a control vector or His-LRRK2G2019S

    Article Snippet: Plasmids were transfected using Roche XTremeGENE HP DNA Transfection Reagent (Sigma-Aldrich, 6366236001) at a 3:1 (ul HP: ug DNA) ratio. siRNA pools targeting human PPP3CB (sc-39196), MCOLN1 (sc-44519), TPCN2 (sc-96434), PRKAA1/AMPKα1-PRKAA2/AMPKα2 (sc-45312), and control siRNA with a scrambled sequence (sc-37007) were purchased from Santa Cruz Biotechnology. siRNA was prepared at 40 nM with 2 μl transfection reagent and transfected overnight before assay. siRNA KD were validated at the protein level.

    Techniques: Translocation Assay, Imaging, Expressing, Control, Plasmid Preparation, Western Blot, Transfection